Reuse of Pleurotus ostreatus contaminated material

In the cultivation of Pleurotus ostreatus, the culture materials are often discarded by infectious bacteria after inoculation due to serious environmental pollution, inadequate disinfection of mushroom houses, and aging of bacteria. In order not to waste raw materials, the following two methods can be used to treat contaminated materials:

Pasteurization method: Use 6-7 kg of lime and 400-500 grams of 25% carbendazim per 100 kg of polluting material, and adjust the polluting material to 65% water content. After mixing well, pile the material as high as possible, use a 4 cm thick wooden stick to punch holes vertically to the bottom, punch holes every 30-40 cm, and pull out by rotation. After the beating, the mulch is fermented and turned over when the temperature of the material rises to 60 ° C to 70 ° C. After re-stacking, the stack was turned twice more according to the previous method, and then the heap was cooled down and the viable strains were introduced. The amount of seed used was about 15%.

Atmospheric pressure sterilization method: first dry the contaminated material, and rake it with a rake for 2 to 3 days. According to 100 kg of polluting material, 3 kg of lime, and 300 g of carbendazim, adjust the water content to about 60%, and sterilize under normal pressure for 10-12 hours after bagging, and then stuffy for 12 hours, and then cool to normal temperature Then, insert the appropriate age strain.

After the inoculation, the temperature of the culture room was maintained at 15 ° C to 20 ° C, and the mycelium was able to develop normally. The bag could be filled in about 35 days, the mushroom buds appeared in 40 days, and the mushrooms could be collected in 4 to 5 days. The development time is 5 to 7 days later than normal, and 2 to 3 tide mushrooms can be collected, and the biological efficiency is 70% to 80%.

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