Enzyme-linked immunosorbent assay (ELISA) for diagnosis of cysticercosis
At present, the new detection methods developed by the laboratory are mainly ELISA and modified ELISA. The cysticercosis antibodies were detected in parallel by five different modified ELISAs: ABC-ELISA, classical ELISA, SPA-ELISA, rapid ELISA and BA ELISA. The results were not significantly different, indicating that the methodological selection was mainly based on habit and experimental purposes.
N. S. Odashima (2002) used ELISA to diagnose the types of antibodies in serum of different patient groups, and found that the ELISA-lgG sensitivity was high, reaching 88.5% and 93.2%, respectively, indicating that the method is fully applicable to porcine cysticercosis. Immunological testing.
Yin Zhujun et al (2003), Yang Baozhen et al (2004) prepared monoclonal antibodies against Cysticercus sinensis for ELISA to detect circulating antibody CAg of cysticercosis, and obtained good results; due to the difficulty in preparation of monoclonal antibodies, new experimental methods were established one after another, and Achieve some results.
Xiang Li (2003) applied cystic fluid antigen to indirectly detect cysticercosis circulating antibodies, which simplifies the steps of preparing monoclonal antibodies and obtains good results. Ma Aixin and other preparations were used to prepare rabbit anti-Swine cysticercosis immunized rabbit serum. Enzyme-linked immunostaining (ELIB) was used to detect circulating antigen in patients with cerebral cysticercosis, which provided a basis for establishing a diagnostic method for cerebral cysticercosis that is sensitive, specific, economical and convenient for practical application.
Wu Jing et al (1999) used Dot-ELISA to detect cysticercosis sensitivity of 98%, specificity of 100%; Nanjing Military Region Joint Military Medical Research Institute developed the porcine cysticercosis circulating antigen enzyme-linked immunoassay kit, after trial Verification shows that its stability and high efficiency meet the requirements (Yang Nan et al., 2004).
R. Biswas (2004) evaluated the Dot-ELISA method. The results showed that the sensitivity was 56.25% and the specificity was 92%. This method proved to be simple and easy to operate, and it is expected to be used in the laboratory diagnosis of pathogens.
Lin X (1999) and other rabbits secreted three different molecular mass proteins secreted by Cysticercus cellulosae to obtain serum antibodies, and the circulating antigen was detected by sandwich EIASA method. The results showed that antiserum was detected by antigen immunized with 53 ku. The circulating antigen has high sensitivity and specificity and can be used for the diagnosis of porcine cysticercosis.
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