Pipette use and maintenance precautions

Many people who use pipettes for the first time always think that the operation of the pipette is very simple, but it is not. The operation of the pipette is the first step in scientific testing and is a very basic test operation, but most people are biased towards the operation of the pipette. In fact, the operation of pipettes is a lot of learning. Ask yourself a few questions and you can see if there is any deviation in your operation.

1. Did you have a liquid suction into the piston during your operation?

2. Do you pay attention to ensure that there is no liquid on the outer wall of the nozzle when you pipette?

3. After using the pipette, have you adjusted the pipette range to the maximum value?

4. After using the pipette, have you placed the pipette flat on the table?

5. Is the pipette operating the same way when moving a thick liquid and a normal liquid?

6. How many pipetting methods does the pipette have?

Pipette use and maintenance considerations:

(1) Setting capacity

When setting the required capacity, it should be adjusted to 1/4 turn larger than the set capacity value, and then adjusted to the set value. This can eliminate the mechanical clearance and make the set value accurate, that is, first rotate the capacity knob beyond the purpose. 1/4 turn of the capacity value and then down to the set value.

(two) the operation of aspiration

1. Connect the appropriate nozzle;

2. Press the control button to the first gear;

3. Place the pipette nozzle vertically into the liquid surface 1-6mm (depending on the size of the pipette);

A pipette of 0.1-10 ul capacity enters the liquid surface 1-2 mm

2-200ul capacity pipette into the liquid surface 2-3mm

1-5ml capacity pipette into the liquid surface 3 -6mm

Note: In order to make the measurement accurate, the nozzle can be pre-washed 3 times, that is, the liquid is repeatedly sucked and discharged 3 times.

4. Slowly slide the control button back into place; ---! ! !

5. Wait for 1-3 seconds for the pipette to move out of the liquid;

Pause for 1 second below 1000ul

5-10ml pause for 2-3 seconds.

6. Slowly remove the nozzle to ensure that there is no liquid on the outer wall of the nozzle.

(three) operation of draining

1. The nozzle is pressed against the inner wall of the capacity at an angle;

2. Slowly press the control button to the first gear and wait for about 1-3 seconds;

3. Press the control button to the second step, the nozzle will drain the remaining liquid;

4. Slow release control button;

5. Press the ejection button to project the nozzle.

(4) Maintenance

1. If the liquid accidentally enters the piston chamber, the pollutants should be removed in time;

2. After the pipette is used, adjust the pipette range to the maximum value and place the pipette vertically on the pipette holder;

3. All pipettes should be cleaned regularly with soapy water or with 60% isopropanol according to the frequency of use, then washed with double distilled water and dried;

4. Avoid placing it at a higher temperature to prevent deformation or leakage or inaccuracy;

5. Find problems and find professionals in time.

(5) Precautions

1. When the pipette nozzle has liquid, do not place the pipette horizontally or upside down to prevent liquid from flowing into the piston chamber to corrode the pipette piston;

2. Correctly use the pipette to aspirate and drain to achieve high precision. Pay particular attention to the drainage 2). 3) Operation;

3. Usually check whether the liquid leaks: After absorbing liquid, stop in the liquid for 1-3 seconds to observe whether the liquid level in the suction head is down; if the liquid level drops first, check if there is any problem with the suction head. If there is any problem, replace the suction head and replace it. The liquid level still drops after the tip indicates that there is a problem with the piston assembly and should be repaired by a qualified service technician.

4. Pipettes that require high temperature disinfection should first consult whether the pipette used is suitable for high temperature sterilization before disposal.

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