Muddy artificial propagation technology
Mudworms have a good ability to adapt to the environment, a wide range of feed sources, and can be cultured in ponds and paddy fields. This is a good project for rural family farming. Muddy natural spawning is longer, usually from April to August each year. From late May to late June, when the water temperature reaches around 20-25°C, it is the spawning period. In order to breed the inbreeding, we must choose the second-in-a-kind, better maturity, strong physique, and no disease-free mud. Female loach is best selected individuals with a body length of 15 cm or more, a body weight of 20 g or more, an abdomen enlargement, a wide pectoral fin, and a rounded front end; a male loach should have a body length and weight slightly smaller than those of female loach, narrow pectoral fin, and pointed tip. The breeding ponds should be pre-repaired, disinfected with quick lime, and then filled with fresh water. Because muddy eggs are not sticky and easy to fall off, fish boxes should be set inside the breeding pond so that after a large number of eggs fall off, they sink into the bottom of the bundle box and they can also effectively hatch the muddy seedlings. Fish nests can be replaced with brown chips, willow twigs or aquatic plants. The types and doses of oxytocic acid used were calculated according to each female loach: 1 for carp pituitary gland, 100-150 international units for human chorionic gonadotropin, or 5-10 micrograms for LRH-A. The loach dose is halved. The solvent injection volume was 0.1-0.2 ml and the syringe was injected using a 1 ml syringe and a 4 gauge needle. The injection site is the abdominal midline, between the pectoral and pelvic fins, but also back muscle injection. Due to muddy bodies, wet gauze can be used for injection (to expose the injection site). After injection, they are placed in the hoppers in the breeding ponds for natural mating and spawning. The proportion of males to females is 1:2. When the water temperature is between 20-25°C, female ovipositors will spawn for 10-20 hours. If artificial insemination is used, the male and female loaches that have been injected with the oxytocculum may be separately raised in small ponds or cages. When the water temperature is 20-25° C., after 10-15 hours, the eggs of the male and female loach abdomen are shed by the light pressure. Artificial insemination. There are two kinds of sperm extraction methods: one is to lightly press the male loach to have semen out of the abdomen; the other method is to remove the testes if the male loach can't squeeze the semen or the semen is not enough, and then wash out the sperm with normal saline. For fertilization. Muddy propagation usually uses dry method for artificial insemination, ie, mixing “dry†semen and eggs (not to be directly exposed to sunlight) in a container, stir evenly with feather or hand, add water and then gently stir for 1-2 minutes to complete artificial insemination. Go to the nest. To nest: Put the fish nest in the hopper of the breeding pond. Then one person gently shakes the fish nest while stirring the water body. The other person slowly pours the fertilized egg into the nest so that the granules are evenly distributed on the nest. After the nest, the fertilized eggs enter the normal incubation stage. Mud-fertilized eggs can normally hatch in the water temperature range of 20-30°C, and generally the seedlings can be hatched in about 2 days. A 0.1 mg/l malachite green solution can be used for disinfection during hatching to prevent egg parasitism.
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