Grapes should be re-emerged before spring

Before the germination of the grapes, the fertilizer is applied in the first month before the germination. The high-quality organic fertilizer and the complete fertilizer of NPK, the appropriate supplement of magnesium fertilizer and phosphorus and potassium fertilizer, the trees of three years or more, each planting and decomposing human and poultry manure 8 ~10 kg, 0.25 kg of NPK (15-15-15) compound fertilizer, 0.15 kg of magnesium sulfate, 1 to 1.5 kg of superphosphate or calcium magnesium phosphate, 0.25 kg of potassium chloride or potassium sulfate. After the application, the fertilizer will be covered, and then 50 to 60 kilograms of water or a mature human or animal manure or biogas slurry will be drenched.

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Nucleic Acid (DNA/RNA) Extraction Kit

1. Introduction

The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:

1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.

2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.

3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.

4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.

3. Shelf life

Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.

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