Gene Technology Improves Traditional Tobacco Industry
A few days ago, the project “Research on the Identification and Application of Chitinase Gene Transgenic Tobacco to Fungal Diseases†undertaken by the Tobacco Research Institute of China Academy of Agricultural Sciences passed the results appraisal organized by the State Tobacco Monopoly Administration. Experts at the meeting believed that the study was designed with reasonable results and reliable results. It is systematic and clearly innovative. As a high-tech reserve for tobacco germplasm improvement, it has potential economic importance for the future development of tobacco production in China, especially for genetically modified tobacco and products. Identification has important business value. For the first time, the chitinase gene from baculovirus was constructed on an expression vector and introduced into tobacco. PCR and Western Blot were used to detect the chitinase gene. The transgenic tobacco line with chitinase gene was obtained. The results of the assay showed that the exogenous chitinase gene was expressed in tobacco and produced biological activity, and the chitinase activity of the transgenic line was higher than that of the non-transgenic parent. This project studied in detail the resistance of transgenic lines to tobacco brown spot disease and anthrax, and obtained a number of tobacco cultivars resistant to disease and transferring chitinase genes; disease-resistant transgenic lines by PCR and Western Blot The test showed genetic stability. This is the first application of the gene in cultivated crops, which has explored a new way for the prevention and control of tobacco fungal diseases. In view of the international tobacco technology barriers and the need of China's foreign trade in tobacco, the identification method of transgenic tobacco was also studied. Semi-nested PCR is a rapid and sensitive method for the detection of transgenic chitinase gene tobacco plants and their modulation. After the new method of tobacco leaves. In addition, the localization of chitinase gene expression in tobacco tissues was also studied by confocal laser scanning microscopy. The results showed that the exogenous chitinase gene is mainly expressed in vascular bundles and accumulated in chloroplasts. The researchers also conducted a preliminary discussion on genetic drift in transgenic tobacco.
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