Effect of circadian rhythm on spontaneous activity in mice

Effect of circadian rhythm on spontaneous activity in mice

Keywords: circadian rhythm; spontaneous activity

We have observed that the number of spontaneous activities of mice varies significantly with different time periods in the day and night, more in the morning, less in the afternoon, more in the evening, less in the middle of the night. The spontaneous activity of mice is often used as an indicator for the study of drug sedation. Therefore, understanding the influence of circadian rhythm on the spontaneous activity of mice is of great significance for the experimental design of drug sedation. For this purpose, we measured the number of spontaneous events in mice at different times.

1 Materials, methods and results

1.1 Material BL 2410 Biological Function Experiment System (Computer), produced by Taimeng Electronics Co., Ltd. Cage (replaced with test tube) 1 only, 1 iron bracket.

1.2 Methods and results

Use a rubber tube for the valve on the bicycle tire to hang the cage on the iron bracket. The lower end of the cage is connected to the tension sensor, the sensor is connected to the computer, and the mouse activity can be recorded on the computer by vibrating the cage. In the experiment, one mouse was placed in the cage for 2m in, and then the number of spontaneous activities of the mouse was recorded for 15 seconds. Computer-recorded analysis of spontaneous activity fluctuations in mice [1]. We measured the number of spontaneous events in mice at seven different times during the day and night. The results were statistically processed in a statistically significant comparison of multiple groups of mean comparisons - the contrast limit method [2]. The number of spontaneous activities in mice was the highest at 9 o'clock in the day and the least at 15 o'clock, the difference was significant (P < 0105), and returned to the highest level at 9 o'clock in the day at 20 o'clock in the evening, and dropped sharply after 2 hours or 22 o'clock. After 2 hours, it will drop faster and reach the lowest point by 24 o'clock. The number of spontaneous activities in mice was significantly different between 20 and 22 (P < 0105), and the difference between 22 and 24 was very significant (P < 0101).

2 Discussion

When we do pharmacological experiments in general, the measurement of experimental indicators is generally based on the experimental group, one group is measured and the other is measured. In the morning, the afternoon measurement is not completed, the afternoon measurement is not completed, or the task is overtime. . This can be arranged for general pharmacological experiments.

The experimental results show that the spontaneous activity of mice is related to circadian rhythm. Therefore, the pharmacological experiment design of sedatives should take into account the circadian rhythm, that is, when measuring the experimental indicators, it is not appropriate to measure one group and then another group. The groups should go hand in hand, that is, each test group should have the same amount. animal. In this way, the influence of the circadian rhythm on the experimental indicators is evenly distributed to each experimental group, thereby avoiding systematic errors caused by group-by-group measurement of each experimental group, especially when measuring at night.