Study on Hybrid Breeding Techniques between Japanese Abalone and Haliotis discus hannai
At present, there are many problems in the production of Haliotis discus hannai, which are characterized by serious degradation of germplasm, long growth cycle, low survival rate, and poor disease resistance, especially in recent years. Virus and bacterial diseases have spread in large areas. The death of youngsters caused a serious impact on the sustainable development of the production of aquiculture. The reasons for the analysis are mainly that the species of abalone used in artificial rearing often come from the same sea area. After many years of breeding, their kinships are getting closer and closer, resulting in inbreeding recession, resulting in serious degradation of germplasm. Therefore, in order for the aquaculture industry to develop steadily, we must do a good job in the optimization and rejuvenation of germplasm. The use of hybrid technology for germplasm improvement and the development of stable, fast-growing and disease-resistant abalone are key to this industry. To this end, since 1999, we have conducted cross-breeding breeding studies between Japanese haliotis and local H. discus hannai in nursery companies such as the nursery of the Jinshan Fisheries Company of Rongcheng City. Good effect. The current study of 1999-2000 is summarized as follows:
1 Materials and Methods
1.1 Materials
1.1.1 Pro-Boo Source
In late January 1999, 1,000 progenitors of abalone were purchased from Changhai County, Dalian, and the specifications were more than 8cm, including 450 females and 550 males. On February 13, 1999, 50 heads of Japanese abalone were purchased from the Fisheries Research Institute of Dalian City, with specifications of more than 10cm, including 22 females and 28 males. On January 20th, 2000, they purchased from Qingdao. More than 1500 heads are 8cm in size, including 980 females and 520 males. On January 15, 2000, 120 heads of Japanese abalone were purchased from Aomori Prefecture, Japan. The specifications were 8.5cm, including 58 females and 62 males.
1.1.2 Bait larvae are cultured with benthic diatoms and artificially-combined baits, fresh seaweeds and kilts of Lai.
1.1.3 Test equipment to stimulate spawning 30W quartz UV lamps, sinks and picking plate. The cages and black corrugated plates were used to breed in the middle of the hatchery. Develop sea trusses and raise cages.
L.2 method
1.1.2 Feed cultivation The cultivation pond and seedling board were first cleaned and disinfected. In the first 1 year of November, benthic diatoms were inoculated and gradually expanded. The expansion was completed by the beginning of April of the second year. Change water 2-3 times a day, each time 1/2 ~ / 3, water temperature to maintain 10 ~ 15 °C, regular fertilization (in conjunction with changing water), nitrogen, phosphorus ratio of 10:1, appropriate amount of iron, silicon fertilizer Wait. To prevent the growth of large diatoms, light is adjusted within the range of 2500 to 3000 lx. Invert the direction of the picking plate regularly to promote uniform breeding and growth of the bait. When scratching on the seedlings plate, the trichlorfon species was killed by adding 1 to 210-6 to the culture pool.
1.1.2 Pro-Braize Storage Pro-brew Pro-Bao Since the beginning of February into the pool to promote cooked, cultivate density 30-50 / m3, pro-Bao into the pool daily warming 1 ~ 1.5 °C, rose to a constant temperature of 20 °C cooked, to be produced. Change the amount of water once a day, change the water temperature does not exceed 0.5 °C, the latter does not exceed 0.3 °C, combined with the removal of dirt, feces observed gonadal development. Every day after the end of changing water, new feeds are put into use, mainly fresh kelp. The daily feeding amount is generally 30% of the weight of the pro-Bal, and it is adjusted at any time according to the water temperature and feeding conditions. In order to improve water quality, increase dissolved oxygen and form water flow, which is conducive to the development of gonads, it is necessary to continuously inflate throughout the day during accelerating, with an aeration rate of not less than 1m3/hour. Bao has a backlight, dark light or dark state is beneficial to the pro-Bao's feeding and activities, so the general lighting control is below 100lx. To maintain a constant room temperature, when the effective accumulated temperature reached 300 ~ 400 °C, pick out the female and male abalone, respectively, promote temporary holdings, daily effective accumulation of temperature and feeding records.
1.2.3 Egg collection, fertilization and hatching
On April 12, 1999, the stored crab abalone naturally spawned at an effective accumulated temperature of 800°C. When the effective accumulated temperature of Japanese abalone reached 1,250°C, it was discharged after artificial stimulation. On May 3, artificial stimulation was used to promote spawning and sperm fertilization in abalone and abalone. On May 15th and June 2nd, inducing spawning and sterilizing of Japanese abalone and abalone discus abalone were also induced by reverse stimulation. The collected eggs were quantified and quantified. After the eggs were settled, the upper clear water was drained. Then about 10,000 eggs were each added to the fertilization. The seawater was stirred while stirring, and the fertilization was completed after half a minute. The eggs were washed several times with fresh filtered seawater. To remove extra sperm. Then, the fertilized eggs were transferred to hatching ponds, and the oviposition density was 20-30/ml. The water was changed every 6-8 hours before the floating of the embryo body, each time was 3/4, and the water temperature was maintained at 18-20°C.
1.2.4 Pre-cultivation of young seedlings and juvenile hatchlings The larvae of the late-stage larvae will be distributed between 10,000 and 15,000 per basket, and the attachment will be completed after 3 days. The seedling-attached rate will be about 30%. 200 to 300. Early nurturing of bait-bao ensures good water quality. The water is changed 3 times a day. Each time the exchange volume is more than 8 times that of cultivating the water body, the water flow in the early stage should be gentle. The water temperature is 18-20°C. In order to ensure the proliferation of benthic diatoms and guarantee the supply of juvenile bait, daily exchange of water is applied every morning with nitrogen 110-6 and phosphorus 0.110-6. Illumination is controlled at 1500 to 3000 lx.
1.2.5 After the young larvae have been cultivated for about 35 days after seedling attachment, and when the size of the young larvae reaches 2 to 3 mm, they are gradually stripped and transferred to cages for cultivation. The peeling method was anesthetized with 2% alcohol seawater. After stripping, the water flow is often 24 hours a day, and the flow rate is more than 10 ranges. Artificially fed diets were fed before the evening of the day. The amount of feed was adjusted at any time depending on the feeding conditions of the youngsters. Adhere to cleanup every morning, remove residual bait and dead seedlings, and periodically pour the pool to wash the cage. About 2 months after the hatchery box, the average shell length is about 9mm, due to the large differences between individuals, at this time to carry out screening and grading, use 2 mesh screen, 1 time to separate 3 body length specifications, Large, medium, and small individuals are kept separately.
1.2.6 Intermediate breeding of abalone The water temperature in November of the year was 6-8°C, peeling screening shell length <1cm, shell length 1～1.5cm and 1.5～2cm, 2～2.5cm and 3cm above 5 specifications. The indoor cement pool is bred in the middle of winter. The middle breeding density is related to the size of the seedlings: shell length <1cm, middle breeding density 3000 ~ 4000/m2, shell length 1 ~ 1.5cm, density 2000 ~ 2500/m2, shell length 1.5 ~ 2cm, density 1000 ~ 1500 The length of the shell is 2 to 2.5 cm, the density is 750 to 1000/m2, and 2.5 to 500 to 750/m2. When Bao Miao enters the pond, it warms up to 1°C per day and rises to 7.8°C. The water is cultivated at a constant temperature. After seawater is warmed up, it is used circularly, and the circulation volume is more than 8 times per day. Every day from 4:00 to 5:00 feeding, the feeding amount of seaweed is 10% to 20% of the body weight of abalone. According to changes in water temperature and the status of juvenile abalone at any time to regulate the amount of feeding, to avoid feeding too much, a waste of food, affecting water quality. When the abalone was found not to be on the board and there was a death, the corrugated board and the cage were replaced immediately. The replaced equipment and tools were disinfected with 5010-6 potassium permanganate and used after sterilization for 2 to 3 days after sun exposure. When the disease is found, the baitiao is fed with a bait or a medicinal bath. The medicine bait uses oxytetracycline and other drugs. After the drug is dissolved, it is mixed into the bait and fed. It takes 2 to 3 days for a course of treatment. The tetracycline and streptomycin are used for the medicinal bath. And so on, generally around 510-6, the medicine bath time is at least 6 hours, and the medicine bath is inflated.
2 results
2.1 In 1999, the survival rate of hybridized seedlings was 40% from attachment to exfoliation (2 to 3 mm). After peeling and cultivating, when the seedlings size is more than 1cm, they are peeled off again, the screening and grading are performed, and the survival rate reaches 60%. After several times of screening and grading cultivation, 6 million hybrid seedlings with a size of 1 cm or more were cultured, and 4286 seedlings emerged as a unit of water. In the 1999 experiment, it was demonstrated that the hybrids of H. discus hannai and H. discus hannai could develop a new generation of seedlings with heterosis, which was significantly better than that of abalone cultivated in self-incompatibility with abalone. Orthogonal crease (♀) x day (♂) combination is best.
2.2 In 2000, the survival rate of the hybrid seedlings was 45% from the attachment to the stripping (2~3mm). After stripping and then cultivating, when the seedling size reaches 1.0 cm or more, it will be peeled again, screened and graded, and the survival rate will be 65%. From the middle of July, more than 2 million small-scale seedlings will be propagated at the bottom of the seedlings, and more than 1 cm of product will be produced. 6 plus ten thousand grains.
2.3 Hybridization group (orthogonal and reversed) and self-crossing group from fertilization to 2～3mm
Comparison of stripping conditions (see Table 1)
From Table 1, it can be seen that the fertilization rate and hatching rate in the hybridization group and the self-crossed group are basically the same, but the seedling survival rate and the seedling survival rate differ greatly. Among them, the orthogonal wrinkle (♀) day is more likely to be reversed. The hybrid effect of wrinkles is better. From the attachment to 2 to 3 mm peeling, the hybridization group showed obvious advantages of high seedling survival rate and survival rate. 2.4 survival rate after the stripping and the survival rate of the middle of the winter to compare to mid-August 1999, Bao Miao grow to more than 1.5cm began to peel screening.
Only 10 pools of hybrid seedlings were screened for 49.47 million grains, and the survival rate was as high as 66.94%, of which 1 cm or more accounted for 45.79%. The self-inflicted group, after the first stripping, entered Zhongwen to cultivate 280,000 seedlings. Only 19,400 tablets were left until the second stripping screening. The survival rate was only 6.21%, and the specification was more than 1cm, accounting for only 13.79%. From the survival rate after overwintering, the survival rate of hybrid seedlings was 95%, while self-crossing was 70%, a difference of 25%. 2.5 Growth rate of hybrid group and self-cross group From the time of hatching until mid-July, the difference in growth speed between hybrid group and self-crossed group was not obvious. From the middle of July to the middle of winter, the average growth speed of the hybrid group was faster than that of the self-crossing group, and the difference was about 0.5cm. Before the sea was cultivated, the average shell length of the hybrid group was longer than that of the self-crossing group by 0.8. Cm or so.
3 Discussion
3.1 Practice has shown that Japanese dish abalone with local wrinkles abalone and abalone as long as the appropriate abalone is suitable in size and well-stocked, can achieve synchronized spawning and sperm production, and can be successfully fertilized and hatched to develop a hybrid generation, regardless of orthogonality or reversal. The purpose of cross breeding can be achieved. Based on the biological zero and maturation temperature of the two, we controlled the rate of warming and the number of days of ripening, so that they can synchronize spawning and ejaculation. 3.2 After two years of experimental production, we found that the offspring produced by the cross combination, especially the offspring produced by the orthogonal combination wrinkle (有ç€), have obvious resistance in terms of disease resistance, growth rate, and survival rate. Advantage. The inbreds showed degradation on the germplasm due to inbreeding. Therefore, breeding new varieties through hybridization is an important way for germplasm to optimize rejuvenation. Practice has shown that 30 to 60 days after childbirth is the peak of death. Because of the mechanical damage of the detachment and the high incidence of disease at this time, many of the self-bred progeny of the family of abalone have poor resistance to disease. After 30 to 60 days of detachment, the mortality rate is high, and the survival rate to the second stripping screening is high. Even less than 10%. Our own self-test also proved this, and the survival rate of the hybrid combination was as high as 60%, and its disease-resistance advantage was obvious. In the sea-cultivation stage, the seedlings raised by the hybrid combination were significantly superior to the self-crossing combination in terms of disease resistance and growth rate, relatively reducing the production cost and shortening the cultivation and breeding cycle.
3. The 32-year experiment proved that the offspring produced by the cross between the native Haliotis discus hannai and Japanese disk abalone was significantly better in traits than the self-inseed abalone seedlings, but the cross-breeding abalone had to be updated annually. In other words, the hybrid superiority could not be found in sub-two. On behalf of the body stable performance. The use of hybrid means to further purify rejuvenation is a very complex project. It is necessary to go through several generations of genetic methods such as self-crossing and backcrossing in order to obtain germplasm with excellent traits. This requires the use of molecular biology methods and long-term research. Therefore, we will carry out this cross-breeding technology for industrialized production promotion and create economic benefits. We will continue to conduct research on the crossbreeding of Bao with joint universities and institutes; we hope to find new varieties with better traits and stability. .
1 Materials and Methods
1.1 Materials
1.1.1 Pro-Boo Source
In late January 1999, 1,000 progenitors of abalone were purchased from Changhai County, Dalian, and the specifications were more than 8cm, including 450 females and 550 males. On February 13, 1999, 50 heads of Japanese abalone were purchased from the Fisheries Research Institute of Dalian City, with specifications of more than 10cm, including 22 females and 28 males. On January 20th, 2000, they purchased from Qingdao. More than 1500 heads are 8cm in size, including 980 females and 520 males. On January 15, 2000, 120 heads of Japanese abalone were purchased from Aomori Prefecture, Japan. The specifications were 8.5cm, including 58 females and 62 males.
1.1.2 Bait larvae are cultured with benthic diatoms and artificially-combined baits, fresh seaweeds and kilts of Lai.
1.1.3 Test equipment to stimulate spawning 30W quartz UV lamps, sinks and picking plate. The cages and black corrugated plates were used to breed in the middle of the hatchery. Develop sea trusses and raise cages.
L.2 method
1.1.2 Feed cultivation The cultivation pond and seedling board were first cleaned and disinfected. In the first 1 year of November, benthic diatoms were inoculated and gradually expanded. The expansion was completed by the beginning of April of the second year. Change water 2-3 times a day, each time 1/2 ~ / 3, water temperature to maintain 10 ~ 15 °C, regular fertilization (in conjunction with changing water), nitrogen, phosphorus ratio of 10:1, appropriate amount of iron, silicon fertilizer Wait. To prevent the growth of large diatoms, light is adjusted within the range of 2500 to 3000 lx. Invert the direction of the picking plate regularly to promote uniform breeding and growth of the bait. When scratching on the seedlings plate, the trichlorfon species was killed by adding 1 to 210-6 to the culture pool.
1.1.2 Pro-Braize Storage Pro-brew Pro-Bao Since the beginning of February into the pool to promote cooked, cultivate density 30-50 / m3, pro-Bao into the pool daily warming 1 ~ 1.5 °C, rose to a constant temperature of 20 °C cooked, to be produced. Change the amount of water once a day, change the water temperature does not exceed 0.5 °C, the latter does not exceed 0.3 °C, combined with the removal of dirt, feces observed gonadal development. Every day after the end of changing water, new feeds are put into use, mainly fresh kelp. The daily feeding amount is generally 30% of the weight of the pro-Bal, and it is adjusted at any time according to the water temperature and feeding conditions. In order to improve water quality, increase dissolved oxygen and form water flow, which is conducive to the development of gonads, it is necessary to continuously inflate throughout the day during accelerating, with an aeration rate of not less than 1m3/hour. Bao has a backlight, dark light or dark state is beneficial to the pro-Bao's feeding and activities, so the general lighting control is below 100lx. To maintain a constant room temperature, when the effective accumulated temperature reached 300 ~ 400 °C, pick out the female and male abalone, respectively, promote temporary holdings, daily effective accumulation of temperature and feeding records.
1.2.3 Egg collection, fertilization and hatching
On April 12, 1999, the stored crab abalone naturally spawned at an effective accumulated temperature of 800°C. When the effective accumulated temperature of Japanese abalone reached 1,250°C, it was discharged after artificial stimulation. On May 3, artificial stimulation was used to promote spawning and sperm fertilization in abalone and abalone. On May 15th and June 2nd, inducing spawning and sterilizing of Japanese abalone and abalone discus abalone were also induced by reverse stimulation. The collected eggs were quantified and quantified. After the eggs were settled, the upper clear water was drained. Then about 10,000 eggs were each added to the fertilization. The seawater was stirred while stirring, and the fertilization was completed after half a minute. The eggs were washed several times with fresh filtered seawater. To remove extra sperm. Then, the fertilized eggs were transferred to hatching ponds, and the oviposition density was 20-30/ml. The water was changed every 6-8 hours before the floating of the embryo body, each time was 3/4, and the water temperature was maintained at 18-20°C.
1.2.4 Pre-cultivation of young seedlings and juvenile hatchlings The larvae of the late-stage larvae will be distributed between 10,000 and 15,000 per basket, and the attachment will be completed after 3 days. The seedling-attached rate will be about 30%. 200 to 300. Early nurturing of bait-bao ensures good water quality. The water is changed 3 times a day. Each time the exchange volume is more than 8 times that of cultivating the water body, the water flow in the early stage should be gentle. The water temperature is 18-20°C. In order to ensure the proliferation of benthic diatoms and guarantee the supply of juvenile bait, daily exchange of water is applied every morning with nitrogen 110-6 and phosphorus 0.110-6. Illumination is controlled at 1500 to 3000 lx.
1.2.5 After the young larvae have been cultivated for about 35 days after seedling attachment, and when the size of the young larvae reaches 2 to 3 mm, they are gradually stripped and transferred to cages for cultivation. The peeling method was anesthetized with 2% alcohol seawater. After stripping, the water flow is often 24 hours a day, and the flow rate is more than 10 ranges. Artificially fed diets were fed before the evening of the day. The amount of feed was adjusted at any time depending on the feeding conditions of the youngsters. Adhere to cleanup every morning, remove residual bait and dead seedlings, and periodically pour the pool to wash the cage. About 2 months after the hatchery box, the average shell length is about 9mm, due to the large differences between individuals, at this time to carry out screening and grading, use 2 mesh screen, 1 time to separate 3 body length specifications, Large, medium, and small individuals are kept separately.
1.2.6 Intermediate breeding of abalone The water temperature in November of the year was 6-8°C, peeling screening shell length <1cm, shell length 1～1.5cm and 1.5～2cm, 2～2.5cm and 3cm above 5 specifications. The indoor cement pool is bred in the middle of winter. The middle breeding density is related to the size of the seedlings: shell length <1cm, middle breeding density 3000 ~ 4000/m2, shell length 1 ~ 1.5cm, density 2000 ~ 2500/m2, shell length 1.5 ~ 2cm, density 1000 ~ 1500 The length of the shell is 2 to 2.5 cm, the density is 750 to 1000/m2, and 2.5 to 500 to 750/m2. When Bao Miao enters the pond, it warms up to 1°C per day and rises to 7.8°C. The water is cultivated at a constant temperature. After seawater is warmed up, it is used circularly, and the circulation volume is more than 8 times per day. Every day from 4:00 to 5:00 feeding, the feeding amount of seaweed is 10% to 20% of the body weight of abalone. According to changes in water temperature and the status of juvenile abalone at any time to regulate the amount of feeding, to avoid feeding too much, a waste of food, affecting water quality. When the abalone was found not to be on the board and there was a death, the corrugated board and the cage were replaced immediately. The replaced equipment and tools were disinfected with 5010-6 potassium permanganate and used after sterilization for 2 to 3 days after sun exposure. When the disease is found, the baitiao is fed with a bait or a medicinal bath. The medicine bait uses oxytetracycline and other drugs. After the drug is dissolved, it is mixed into the bait and fed. It takes 2 to 3 days for a course of treatment. The tetracycline and streptomycin are used for the medicinal bath. And so on, generally around 510-6, the medicine bath time is at least 6 hours, and the medicine bath is inflated.
2 results
2.1 In 1999, the survival rate of hybridized seedlings was 40% from attachment to exfoliation (2 to 3 mm). After peeling and cultivating, when the seedlings size is more than 1cm, they are peeled off again, the screening and grading are performed, and the survival rate reaches 60%. After several times of screening and grading cultivation, 6 million hybrid seedlings with a size of 1 cm or more were cultured, and 4286 seedlings emerged as a unit of water. In the 1999 experiment, it was demonstrated that the hybrids of H. discus hannai and H. discus hannai could develop a new generation of seedlings with heterosis, which was significantly better than that of abalone cultivated in self-incompatibility with abalone. Orthogonal crease (♀) x day (♂) combination is best.
2.2 In 2000, the survival rate of the hybrid seedlings was 45% from the attachment to the stripping (2~3mm). After stripping and then cultivating, when the seedling size reaches 1.0 cm or more, it will be peeled again, screened and graded, and the survival rate will be 65%. From the middle of July, more than 2 million small-scale seedlings will be propagated at the bottom of the seedlings, and more than 1 cm of product will be produced. 6 plus ten thousand grains.
2.3 Hybridization group (orthogonal and reversed) and self-crossing group from fertilization to 2～3mm
Comparison of stripping conditions (see Table 1)
From Table 1, it can be seen that the fertilization rate and hatching rate in the hybridization group and the self-crossed group are basically the same, but the seedling survival rate and the seedling survival rate differ greatly. Among them, the orthogonal wrinkle (♀) day is more likely to be reversed. The hybrid effect of wrinkles is better. From the attachment to 2 to 3 mm peeling, the hybridization group showed obvious advantages of high seedling survival rate and survival rate. 2.4 survival rate after the stripping and the survival rate of the middle of the winter to compare to mid-August 1999, Bao Miao grow to more than 1.5cm began to peel screening.
Only 10 pools of hybrid seedlings were screened for 49.47 million grains, and the survival rate was as high as 66.94%, of which 1 cm or more accounted for 45.79%. The self-inflicted group, after the first stripping, entered Zhongwen to cultivate 280,000 seedlings. Only 19,400 tablets were left until the second stripping screening. The survival rate was only 6.21%, and the specification was more than 1cm, accounting for only 13.79%. From the survival rate after overwintering, the survival rate of hybrid seedlings was 95%, while self-crossing was 70%, a difference of 25%. 2.5 Growth rate of hybrid group and self-cross group From the time of hatching until mid-July, the difference in growth speed between hybrid group and self-crossed group was not obvious. From the middle of July to the middle of winter, the average growth speed of the hybrid group was faster than that of the self-crossing group, and the difference was about 0.5cm. Before the sea was cultivated, the average shell length of the hybrid group was longer than that of the self-crossing group by 0.8. Cm or so.
3 Discussion
3.1 Practice has shown that Japanese dish abalone with local wrinkles abalone and abalone as long as the appropriate abalone is suitable in size and well-stocked, can achieve synchronized spawning and sperm production, and can be successfully fertilized and hatched to develop a hybrid generation, regardless of orthogonality or reversal. The purpose of cross breeding can be achieved. Based on the biological zero and maturation temperature of the two, we controlled the rate of warming and the number of days of ripening, so that they can synchronize spawning and ejaculation. 3.2 After two years of experimental production, we found that the offspring produced by the cross combination, especially the offspring produced by the orthogonal combination wrinkle (有ç€), have obvious resistance in terms of disease resistance, growth rate, and survival rate. Advantage. The inbreds showed degradation on the germplasm due to inbreeding. Therefore, breeding new varieties through hybridization is an important way for germplasm to optimize rejuvenation. Practice has shown that 30 to 60 days after childbirth is the peak of death. Because of the mechanical damage of the detachment and the high incidence of disease at this time, many of the self-bred progeny of the family of abalone have poor resistance to disease. After 30 to 60 days of detachment, the mortality rate is high, and the survival rate to the second stripping screening is high. Even less than 10%. Our own self-test also proved this, and the survival rate of the hybrid combination was as high as 60%, and its disease-resistance advantage was obvious. In the sea-cultivation stage, the seedlings raised by the hybrid combination were significantly superior to the self-crossing combination in terms of disease resistance and growth rate, relatively reducing the production cost and shortening the cultivation and breeding cycle.
3. The 32-year experiment proved that the offspring produced by the cross between the native Haliotis discus hannai and Japanese disk abalone was significantly better in traits than the self-inseed abalone seedlings, but the cross-breeding abalone had to be updated annually. In other words, the hybrid superiority could not be found in sub-two. On behalf of the body stable performance. The use of hybrid means to further purify rejuvenation is a very complex project. It is necessary to go through several generations of genetic methods such as self-crossing and backcrossing in order to obtain germplasm with excellent traits. This requires the use of molecular biology methods and long-term research. Therefore, we will carry out this cross-breeding technology for industrialized production promotion and create economic benefits. We will continue to conduct research on the crossbreeding of Bao with joint universities and institutes; we hope to find new varieties with better traits and stability. .
Natural pigment supplier Organic Herb Inc's range of products include: Marigold Extract (lutein, yellow pigment), Sorghum biocolor extract (Sorghum pigment, red), beetroot extract (Beet Red), Ginger extract (gingerol yellow), Black Carrot Extract (red), Purple Kale pigment(red cabbage pigment), Purple Sweet Potato Color, chili extract (capsicum red pigment), Gardenia Extract (Gardenia Yellow) etc, these Natural Colors can be widely used as food additives , beverage additives and dietary supplement etc.
Natural Pigments have grabbed a lot of attention as an alternative of synthetic pigments.The plant extracted color finds its application in a variety of fields, including pharmaceuticals drug production ,nutraceuticals,food production ,dairy, beverages, animal feed ,cosmic and the confectionery segment.Plant extract of natural colors is not only good for coloring but also good for improve wellness as most of them are antioxidants.Organic Herb Inc is a China-based plant extract manufacturing company which takes pride in providing a wide range of high quality environmentally friendly and water miscible pigments from organic plants at competitive market prices.
Natural Colors,Natural Pigments,Carrot Pigment,Gardenia Extract Powder
Organic Herb Inc. , http://www.organic-extracts.com