Application of Gradiflow Technology in Protein Separation Research

Electrophoresis is a common and effective tool for modern protein research, but the heat generated during electrophoresis destroys the structure of proteins, and the low recovery efficiency of the target protein from protein gel or other media often leads to more in-depth study of proteins. The abrupt end, and the application of Gradiflow technology solves the headache of researchers!

The technical points of Gradiflow can be summarized as follows:

1. Apply polyacrylamide separation membranes of different pore sizes;

2. Separation based on the molecular weight or (and) isoelectric point of the protein or polypeptide;

3. Proteins with different isoelectric points have different charging properties in buffers of different pH;

4. Proteins of different molecular weights pass through separation membranes of different pore sizes.

Gradiflow technology has the following outstanding performance in protein separation studies:

1. Purification of antibodies

In the application of most antibodies, there are certain requirements for purity. Usually, researchers prefer to use purification methods such as precipitation and affinity chromatography, but the source of antibodies often limits the purification work of researchers. However, Gradiflow technology can solve such problems, whether it is polyclonal antibody or monoclonal antibody, whether it is sheep-derived antibody, rabbit-derived antibody, or bovine source, human source and other antibodies, can be purified.

Examples of purification of polyclonal antibodies:

Recovery of Ig from Gradiflow and affinity chromatography

2. Proteome pre-separation

2DE is a proteomic analysis method commonly used by researchers. This method is very useful, but its limiting factors gradually emerge during the experiment. For example, some membrane proteins, high molecular weight and low molecular weight proteins are difficult to detect with 2DE. . In particular, some protein samples contain high-abundance proteins, which makes it possible to mask the proteins of interest to the researchers and not reflect them on the 2DE map, thus reducing the amount of protein detected. After the sample is pre-separated by Gradiflow technology, the above problem can be easily solved by performing the 2DE experiment.

Examples of removal of HAS in plasma using Gradiflow technology under non-denaturing conditions:

NuSep's ProteomeSep MF10, launched in 2008, inherits Gradiflow's technology and complements and improves the entire system to become a new generation of protein pre-separation tools!